Ben Diskin
Numbuh 1 / Numbuh 2 (voice)
Tom Kenny
Goof (voice), Numbuh 30c (voice), Mr. Jelly / Knightbrace (voice), Mr. Wink (voice), Narrator (voice), Twin Boy / Vicious Dog (voice), Kenny (voice), Numbuh 16 / Numbuh 67 / Numbuh 68 (voice), Teen / Jock / Guard (voice), Numbuh 30C (voice), Chester / President / Robber #1 (voice), Hoagie Gilligan IV (voice), The Common Cold / Flu Bug (voice), Knightbrace (voice), Fake Delightful Child from Down the Lane (voice), Numbuh 42 / Numbuh Ezekiel (voice), Counselor / Campers (voice), Chester (voice)
Jennifer Hale
Girls (voice), Numbuh 86 / Nurse Claiborne / Rainbow Monkey Chorus (voice), Mrs. Beetles (voice), Dixie / Runt (voice), Secretary / Assistant (voice), Mrs. Uno / Major Mrs. Manners (voice), Computer / Rupert's Mom / Reporter (voice), Mrs. Thompson / Robber #3 (voice), (voice), Computer (voice), Mrs. Thompson (voice), Stacey (voice), Numbuh 202 (voice), Mrs. Uno (voice), Computer / Nerd (voice)
Daran Norris
RAMON-4 (voice), Maitre'd / Violinist (voice), Count Spankulot / Gooey / Ice Cream Man #2 (voice), Announcer / Assistant / Homeworko (voice), Mr. Physically Fitastic / The Human Text (voice), Ed / Guard #1 / Lead Mustache (voice), Gooey (voice), (voice), Monty Uno / Count Spankulot / Sargent Sensible (voice), Joe Balooka (voice), Guards / DJ (voice), Count Spankulot / Joe Balooka / Spankenstine Monster (voice)
Jeff Bennett
Destructo Dad / Mr. Boss / Mr. Fizz (voice), Sydney Beetles (voice), Numbuh Jebediah (voice), Mr. Boss (voice), Sydney Beetles / Mr. Boss / Technicians (voice), Hoagie Gilligan Sr. (voice), Dr. Teef (voice), Destructo Dad (voice)
Candi Milo
Henrietta Von Marzipan (voice), Betty Gilligan (voice), Leona (voice), Salmonella Fitzgerald (voice), Edna Jucation (voice), Grandma Lydia (voice), Madame Margaret (voice), Nurse Jumbo (voice), Lasso Lass (voice), Leaky Leona / Mrs. Goodwall (voice), Betty Gilligan / Grandma Lydia (voice)
Jason Harris
Numbuh 274 (voice), Commander (voice), Chad Dickson (voice), Marty / Jock (voice), (voice)
Frank Welker
Professor XXXL / Chimpy (voice), Truck Driver (voice), Rainbow Monkey Kong (voice), Professor XXXL / Defense Array (voice), Bus Driver (voice)
Tara Strong
Sally Sanban (voice), Girls (voice), Girl (voice), Mushi Sanban / Stewie (voice), Pauley Marcus (voice), Mushi Sanban / Numbuh 93 (voice), Rainbow Monkey Tribesgirl #3 (voice), Mushi Sanban (voice)
Grey DeLisle
Grace / Lizzie Devine (voice), Lizzie Devine / Laura Limpin (voice), Crazy Old Cat Lady / Mega Mom (voice), Lizzie / Woman (voice), Laura Limpin / Additional Voices (voice), Julie / Cheerleader (voice), Mega Mom / Numbuh 69 (voice), Gramma Stuffum (voice)
Mark Hamill
Stickybeard / Sumo Wrestler / Pirate (voice), Stickybeard (voice)
Kevin Michael Richardson
Ice Cream Man / Ice Cream Beast (voice), Dr. Lincoln (voice)
James Arnold Taylor
Eggbert Eggleston (voice), King Sandy / Knight #2 / Guard #1 (voice)
Rachael MacFarlane
Girls (voice), Numbuh 362 (voice), (voice)
Dave Wittenberg
Nick (voice), Ernest "Ernie" / Nick (voice), Numbuh 74.239 (voice), Wintergreen (voice)
Rob Paulsen
Robin Food (voice), Rupert Puttkin / The Great Puttinski (voice), Mr. Washer / Chili Dog Vendor (voice), (voice), Mr. Mogul / Monkey (voice)
Carlos Alazraqui
Numbuh 2x4 (voice), The Kid / Pedestrian (voice)
Josh Peck
Numbuh 50 Million B.C. (voice)
