Wilson Cruz
Charo (voice), Evan Martinez (voice)
Alan Cumming
Rupert (voice), Chuck (voice), Chuck Masters (voice), Zeke (voice)
Will Matthews
Echinacea / Timmy (voice), Rick Brocka Jr. (voice), Echinacea (voice), Timmy (voice), F. Cornelious Hampton-Hampton III (voice)
Q. Allan Brocka
Flaxseed / Greenpeacer / "We're All Going to Die" Guy (voice), Father Figure / Muddy Frat Boy / Javelin Thrower (voice), Ricky Brocka Sr. / Republican / Hysterical Klansman (voice), Falaysha (voice), Monsterbator Promoter / Announcer / Receptionist / Nerds (voice), Falaysha / Ricky Brocka Sr. (voice), Announcer / Protester / Straight Guy / Club Girl (voice), Frank Nerdlinger / Michaela (voice), Condom Student (voice), Wiener / Ricky Brocka Sr. / Stud #1 / Stud #2 (voice), Ricky Brocka Sr. (voice)
Billy West
Crazy Raul / Straight Guy (voice), Additional Voices (voice), Hunter (voice), Dr. Hunk (voice), Log Cabin Tom / Dylan Rambrick (voice), George W. Bush (voice), Asspounder / Carlton (voice), Carlton (voice), Log Cabin Tom (voice), Ryan Peefest (voice), Anderson Pooper (voice)
Liza del Mundo
Pussy / Ebony / Nerds (voice), Minda / Pussy / Additional Voices (voice), Ebony / Arugula (voice), Migrant Worker (voice), Pussy (voice), Minda / Clever Dead Housewife (voice), Clever Housewife (voice), Pussy / Myrna (voice), Minda / Pussy / Ebony (voice), Arugula (voice), Pussy / Ursula (voice), Minda / Pussy / Clever Dead Housewife (voice)
Margaret Cho
Condie Ling (voice), Condie Ling / Jessica (voice), Condie Ling / Kathy Griffin (voice)
Emily Brooke Hands
Straight Woman / Protestor (voice), Heather (voice), Kirsten (voice), Mrs. Peghole / Rita (voice)
Lori Alan
Ivory / Dixie (voice), Dixie / Mail Woman / Eunice (voice), Ivory / Nerds (voice), Dixie (voice), Dixie / Nerd (voice), Ivory / Dr. Joy / Additional Voices (voice), Dixie / Ivory (voice)
Jessica-Snow Wilson
Kirsten / Mary Jane (voice), Kirsten (voice)
Laraine Newman
Chastity Bono / Announcer / Additional Voices (voice)
Jim J. Bullock
Jacques-Jean / Emerald Joe (voice)
Jill Bennett
General B. Traytus (voice)
Miss Coco Peru
Mother Morally Superior (voice)
George Takei
Mayor Screwsum / Police Chief (voice)
Bruce Vilanch
Bruce Bandersnatch (voice)
Robert Gant
Mayor Mayer / Student (voice)
Heidi Hamilton
Ann Coulter / Rita (voice)
