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Judy Greer

Beep (voice)

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Fred Tatasciore

Bang (voice)

Jeff Gill

Bing (voice)

Gregg Spiridellis

Boop (voice)

Evan Spiridellis

Hap

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Erin Fitzgerald

Bo / Roxy / Eardrum / Stapes (voice)

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Jim Meskimen

Horn Head (voice), Mr. Protein (voice), Additional Voices (voice), Virus King / B-Cell (voice)

Nate Theis

Additional Voice (voice), Hub / Bub (voice)

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'Weird Al' Yankovic

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Jay Leno

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Kevin Smith

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Whoopi Goldberg

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Riki Lindhome

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Kate Micucci

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Tim Meadows

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Chris Parnell

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Snoop Dogg

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Christina Applegate

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Kristen Schaal

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Ali Wong

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David Koechner

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Edward Norton

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David Cross

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Wanda Sykes

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John Legend

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Zoe Saldaña

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Reggie Watts

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Tony Hale

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Jennifer Garner

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Jason Sudeikis

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Alyssa Milano

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Maria Bamford

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Nora King

Helper-T (voice)

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Randy Crenshaw

Macrophage (voice)

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Eric Bauza

Mr. Recorder (voice)

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John DiMaggio

Mr. Sun / Mr. Whale (voice)

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Neil Kaplan

Additional Voices (voice)